Murdock Lab

1. Pricing

On Campus Users:

$ 105.00 full, 96-well plate*. $1.094 each $ 400.00 full, 384-well plate*. $1.042 each $ 1.20 each, run in multiples of 16. $19.20 minimum charge. --The on campus rates are for those PIs who are directly associated with, or collaborating with, The University of Montana as faculty, staff, or student. All others are considered off campus users. Off Campus Users: $ 115.00 full, 96-well plate*. $1.20 each $ 425.00 full, 384-well plate*. $1.107 each $ 1.50 each <96-wells—run in multiples of 16 ($24.00 minimum)

*Murdock Lab will supply plates unless the user wishes to purchase the specialized plates from a vendor. We run fragment analysis on batches of 16, or multiples thereof. Submissions with less than 16 samples will be charged 16 lanes.

2.  Submitting your samples
You can select from three different size standards at this time, depending on the markers you have chosen for your labeled primers.
GS600LIZ – can use markers labeled with 6-FAM (blue), VIC (green), NED (yellow), PET (red).
GS500ROX –can use markers labeled with 6-FAM (blue), HEX (green), NED (yellow).
MM1000ROX—can use markers labeled with 6-FAM (blue), HEX (green), NED (yellow).

Following your PCR, pick up a plate from the Murdock lab, dilute your samples appropriately and aliquot them into the wells.  Each researcher will determine what dilution and aliquot amount to use following optimization.  As a guideline: when using the LIZ600 size, standard, protocol requests .5ul of diluted PCR product per well; and, when using the ROX size standards the request is for 1ul. 
Return your plate with diluted product aliquots to the order drop off fridge and notify the Murdock Lab via email that it is in.
Mailing in your plates – we have found the following protocol works well for mailing in plates.

1. You can order the specific plates (semi-skirted, raised skirt 96 well or 384 well) from any vendor.  Call before purchasing to be sure they will work on the Applied Biosystems 3130xl.  We use the Gold Series Semi-Skirted  96-well pcr plates from ISC-Bioexpress (Bioexpress.com catalog #T-3085-P)—the color allows for better visualization of aliquots.
   
   
2. Aliquot product into wells accordingly.
   
   
3. Spin down the plate briefly to ensure product is seated at bottom of well.
   
   
4. Dry down sample so it does not shift during transit.  One way is to cover the plate lightly with a KimWipe or paper towel to prevent contamination and allow water to evaporate overnight. Please don’t use a Speedvac as this will make it difficult to resuspend.
   
   
5. Seal the plate with a secure plate sealing adhesive cover and ship in a small padded envelope.
   
   
6. Complete the online order form.  Charge cards are preferable, but PO is acceptable.

3. Placing your Order
You will need to “Create an Account” before placing your first order.  This will generate an account name.  The account name will be used for ordering as well as retrieving your results.
Use this website to place your order by selecting the “Place Order” tab above, scrolling down to and clicking on the “Fragment Analysis Order” hyperlink.  After entering your information and selecting the size the appropriate size standard click on “Next” to be taken to a screen that allows you to download an Excel format template.  Complete your template, save it, and then upload it into the form. Click “submit”.
If you have entered your email address correctly on the order form, you will receive a confirmation email.

--If the website is down, contact the facility at (406) 243-4229 and an order sheet will be emailed for your completion.